The ANP/cGMP/cGMP-dependent protein kinase pathway counteracts Angiotensin II-stimulated calcium mobilization in cardiac myocytes
نویسندگان
چکیده
Background The cardiac hormone atrial natriuretic peptide (ANP) exerts local, Guanylyl Cyclase-A (GC-A) – mediated myocardial effects to antagonize the Cai-dependent hypertrophic growth response to Angiotensin II (Ang II). The present study aimed to characterize the specific molecular mechanisms mediating the reciprocal interactions between the ANP/GC-A and the Ang II/AT1 systems in the heart.
منابع مشابه
Expression of guanylyl cyclase-A/atrial natriuretic peptide receptor blocks the activation of protein kinase C in vascular smooth muscle cells. Role of cGMP and cGMP-dependent protein kinase.
To understand the molecular mechanisms of cellular signaling of atrial natriuretic peptide (ANP), we have studied its effect on the enzymatic activity of endogenous and overexpressed protein kinase C (PKC) in rat thoracic aortic vascular smooth muscle (RTASM) cells. Angiotensin II (ANG II), endothelin-1 (ET-1), and 12-O-tetradecanoylphorbol 13-acetate (TPA) stimulated fourfold to fivefold PKC a...
متن کاملReply to the Letter to the Editor
To the Editor roles of guanylyl cyclases must be viewed carefully. The reports [8,9] cited by Dr. Herring, et al. gave evidence We thank Dr. Herring et al. for their comments on our against the endothelial NO synthase hypothesis and NO in paper. In our recent report [1] we proposed that the muscarinic inhibition of I . Such experiments could not postjunctional source of cardiomyocyte cGMP, from...
متن کاملReply to the Letter to the
address completely the issue of guanylyl cyclase and soluble or particulate guanylyl cyclase, imposed direcmuscarinic inhibition. Moreover, we consider that recent tionality on the response to ligands. Such considerations investigations have strengthened rather than diminished the also apply to stimulation of L-type calcium current (I ) Ca(L) importance of cGMP signaling, per se and in connecti...
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The effects of 8-bromo-cGMP on intracellular calcium concentrations in cultured rat aortic smooth muscle cells were studied. Both angiotensin II and depolarizing concentrations of K+ stimulated Ca2+ accumulation in the cytoplasm. The increase in Ca2+ due to angiotensin II was associated with an increase in inositol phosphates, while that due to K+ was not. Preincubation of cells with 8-bromo-cG...
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Atrial natriuretic peptide antifibrotic properties are mainly described in cardiac myocytes or in induced cardiac myofibroblasts (Angiotensin II or TGF-beta induced differentiation). In the present work, we investigate the effects of ANP/NPRA/cGMP system in modulating rat cardiac fibroblasts function. Cardiac fibroblasts were isolated from adult Wistar male rats and cultured in the presence of ...
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